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. Author manuscript; available in PMC: 2020 Nov 30.
Published in final edited form as: Photochem Photobiol. 2012 Feb 17;88(5):1165–1172. doi: 10.1111/j.1751-1097.2012.01097.x

Figure 3.

Figure 3.

RES inhibits mTORC2 activity by downregulation of Rictor expression. Western blot analysis of extracts prepared from A431 cells that were either untreated or treated with 50 μM RES for 48 h or 0.1 μM RAP for 48 h (80 μg extract was loaded per lane). RhoA-GTP bound to Rhotekin-RBD beads and total cellular RhoA was detected by Western blotting using an anti-RhoA antibody. β-actin was used as an internal loading control. The levels of Akt1, p-Akt1, Rictor, PTEN, p-p70S6K, and p70S6K were assessed by Western blot analysis.