RES inhibits mTORC2 activity by downregulation of Rictor expression. Western blot analysis of extracts prepared from A431 cells that were either untreated or treated with 50 μM RES for 48 h or 0.1 μM RAP for 48 h (80 μg extract was loaded per lane). RhoA-GTP bound to Rhotekin-RBD beads and total cellular RhoA was detected by Western blotting using an anti-RhoA antibody. β-actin was used as an internal loading control. The levels of Akt1, p-Akt1, Rictor, PTEN, p-p70S6K, and p70S6K were assessed by Western blot analysis.