Table 2. Summary of disconcordant West Nile and dengue virus neutralizing antibody results between the New York multiplex microsphere assay (NY—MIA) and the University of Texas at El Paso plaque reduction neutralization test (UTEP—PRNT) for 9 of 59 cord-blood plasma samples obtained from mothers at the Providence, Sierra East, Sierra Main hospitals, El Paso, Texas.

			Median Fluorescence Intensity Values of samples reactive with West Nile virus envelope and/or West Nile and dengue virus (DEN) nonstructural antigens								UTEP PRNT80 Titers
	ELISA IgG Antibodies		WNV-E	WNV-NS1	WNV-NS5	Den 1 NS1	Den 2 NS1	Den 3 NS1	Den 4 NS1	NY—MIA	Antibody Titers		PRNT80
Sample Code	DENV	WNV	232*	212	6680	808	746	615	405	Diagnosis antibody	DENV	WNV	Diagnosis antibody
PMH0037	400	1600	1004	171	459	14	10	41.5	17	Flavivirus Envelope	≤20	320	WNV
PMH0167	1600	6400	5523	1048	1300	2498	2630	8207	2944	WNV/DENV	≤20	40	WNV
SES0146	400	100	737	262	2862	488	382	860	345	Flavivirus Envelope	≤20	≤20	Neg
SES0450	400	6400	364	81	629	19	29	20	19	Flavivirus Envelope	≤20	1280	WNV
SES0632	400	6400	620	179	504	19	15	62	31	Flavivirus Envelope	≤20	1280	WNV
SES0654	1600	6400	2343	249	1053	46	53	55	46	WNV	DENV3 (160), DENV4 (640)	WNV (320)	WNV/DENV
SES0827	1600	1600	273	53	658	273	113	461	78	Flavivirus Envelope	≤20	320	WNV
SMC0055	1600	1600	407	30	430	12	10	107	15	Flavivirus Envelope	≤20	≤20	Neg
SMC0119	200	≤100	851	33	331	564	149	310	71	Flavivirus Envelope	≤20	≤20	Neg

*- Median fluorescence intensity cut-off values for test samples equal to or higher representing antigen–antibody reactivity, ELISA cut-off values DENV IgG cut off = 0.24–0.29, WNV IgG cut off = 0.11–0.16, samples positive for both DENV and WNV antibodies with the same antibody titers or less than 4-fold difference = antibody positive to both viruses, virus with 4-fold or greater antibody titer considered antibody positive for the virus with the highest antibody titer. (7 flavivirus and 2 WN and/or DEN)