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. 2020 Nov 30;15(11):e0242827. doi: 10.1371/journal.pone.0242827

Fig 7. Pro-inflammatory gene expressions.

Fig 7

Rats were subjected to fast brain death (BD) induction (intracranial pressure (ICP) increase over 1 min) versus slow BD induction (ICP increase over 30 min). Lungs were procured after 0.5–4 hours of donor stabilization and the following gene expression levels for pro-inflammatory cytokines were assessed by means of RT-qPCR: (A) TNF-α, (B) IL-6, (C) IL-8-like CINC-1, (D) MCP-1, (E) VCAM-1 and (F) C3. Data are shown as expression relative to housekeeping genes PPIA, ELf2b1 and β-actin. Values are presented as mean ± SEM. Asterisks denote significant differences between the two BD models per time point: * p<0.05. ‘#’-symbols indicate significant differences compared to controls. X-axis 0.5–4hours–brain-dead group with period of ventilation and hemodynamic stabilization time; Control–immediately sacrificed without intervention; FM–fast BD induction model, expansion of the Fogarty catheter over 1 min; SM–slow BD induction model, expansion of the Fogarty catheter over 30 min.