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. 2020 Nov 6;9:e61965. doi: 10.7554/eLife.61965

Figure 7. The memory consolidated cells are highly sensitive to low-dose TNF-α induction.

Figure 7.

(A) RT-qPCR results show the CALCB transcriptional level of naïve cells and of cells that had experienced 12 days of TNF-α treatment following 12 hr of 0.4 ng/mL, 2 ng/mL, 10 ng/mL, and 50 ng/mL TNF-α treatments. The cells were cultured in TNF-α-free media for 10 days before receiving a second TNF-α stimulation. GAPDH is used as the internal control. Data are shown as mean ± SD from two independent experiments. Two-tailed t-test. (B) Expression levels (in FPKM) of EGFP, CALCB, and PTGES for naïve cells and memory consolidated cells in response to 12 hr TNF-α induction with various TNF-α concentrations. NFKB2 and NFKBIA, two TNF-α induced genes with no memory effect, are shown for comparison. Data are shown as the mean from two independent experiments. (C) Model illustration for the co-option of an ERV into the transcriptional memory module of CALCB in response to TNF-α. Memory consolidation offers faster, stronger, and more sensitive subsequent induction.

Figure 7—source data 1. Related to Figure 7A.
Figure 7—source data 2. Related to Figure 7B.