Table 2. CryoEM data collection and refinement statistics.
| Data collection | |
|---|---|
| Electron microscope | Titan Krios G3 |
| Camera | Falcon 3EC |
| Voltage (kV) | 300 |
| Nominal magnification | 75,000 |
| Calibrated physical pixel size (Å) | 1.06 |
| Total exposure (e- /Å2) | 42.7 |
| Number of frames | 30 |
| Image processing | |
| Motion correction software | cryoSPARCv2 |
| CTF estimation software | cryoSPARCv2 |
| Particle selection software | cryoSPARCv2 |
| 3D map classification and refinement software | cryoSPARCv2 |
| Micrographs used | 2080 |
| Particles selected | 669,223 |
| Global resolution (Å) | 3.2 |
| Particles contributing to final map | 165,747 |
| Model building | |
| Modeling software | Coot, phenix.real_space_refine |
| Number of residues built | 3085 |
| RMS (bonds) | 0.002 |
| RMS (angles) | 0.56 |
| Ramachandran favored (%) | 95.8 |
| Rotamer outliers (%) | 0.5 |
| Clashscore | 6.27 |
| MolProbity score | 1.63 |
| EMRinger score | 2.54 |