Appendix 2—table 1. Parameters used in the large transport intermediate formation model.

The free energy Equation (2) depends on a number of different parameters, which are described in this table.

Parameter	Description	Value	Notes	Reference
$\sigma$	Membrane tension	0.003 kBT/nm2 (ER); 0.0012 kBT/nm2 (Golgi membranes)		Upadhyaya and Sheetz, 2004
${\kappa }_b$	Membrane bending rigidity	20 kBT		Niggemann et al., 1995
$R_c$	Radius of curvature of the COPII coat	37.5 nm		Miller and Schekman, 2013
${\lambda }_0$	Bare coat line tension	0.012 kBT/nm	Not measured for COPII. Used the clathrin value as a reference	Saleem et al., 2015
${{\mu }_c}^0$	COPII coat polymerization energy	0.024 ± 0.012 kBT/nm2	Not measured for COPII. Used the clathrin values as a reference	Saleem et al., 2015
${\kappa }_T$	TANGO1 filament bending rigidity	120 kBT nm	Not measured. Range based on standard filament rigidities (see text)
$c_0$	TANGO1 filament spontaneous curvature	(−0.02, 0.02) nm−1	Not measured. Range based on observed TANGO1 ring sizes	Raote et al., 2017
$∆\lambda$	TANGO1 linactant strength	0.12 kBT/nm	Not measured. Range based on protein-protein affinity (see text)	-
$N$	Outwards-directed force	0 – 5kBT/nm	Not measured. Range based on known intracellular forces	Kovar and Pollard, 2004 (Actin); Block et al., 2003 (Molecular motors)