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. 2020 Nov 23;55(4):483–499.e7. doi: 10.1016/j.devcel.2020.09.002

Figure 7.

Figure 7

TBX-37/38 Determine Left-Right Molecular Asymmetry across Multiple Bilateral Neuron Pairs with ABa/ABp Lineage Origin

(A) Schematic of the reporter used to monitor expression of C32C4.16 (in the context of a fosmid clone containing ~40 kb of flanking genomic sequence), producing nuclear GFP separated from the C32C4.16 protein product through a 2A peptide (Ahier and Jarriault, 2014). Representative images show a larval head with left-specific expression across ASE neurons (labeled with che-1prom::mCherry) and potentially other neurons on the left side.

(B) C32C4.16 expression is also lateralized across AVE and AUA neuron pairs (labeled with opt-3prom::TagRFP and flp-8prom:: RFP, respectively) (n = 10 per genotype).

(C) Expression of C32C4.16 requires TBX-37/38 and their binding sites for the majority of its expression pattern (n = 20 per genotype). All scale bars represent 10 μm.

(D) Schematic of the lineage origin of the AVE, AUA, and other neuron pairs that were also identified as having asymmetric C32C4.16 expression (Figure S6).