Figure 5.

Effects of paricalcitol on senescence in the kidney after ioversol administration. (a) β-Galactosidase staining was performed with a specific assay kit. Densitometric quantification for β-galactosidase was applied to each group. Positive signals for β-galactosidase were observed as blue in the ioversol-treated kidney. Images are representative of each group. (b) Immunoblot analysis was performed with a specific antibody against p16INK4A. β-Actin was used as loading control, and data were normalized against the density of β-actin. Blots are representative of each group. Cropped blots are displayed here, and full-length blots are included in the section of Supplementary Information. Con: PBS-treated group; PC: paricalcitol-treated group; CONT: ioversol-treated group; PC+CONT: paricalcitol- and ioversol-treated group. Data were presented as mean ± SEM (^∗P < 0.05).