Fig. 2.

Effect of TGF-β on protein expression of Lin28a and renal fibrotic factors. (A) Western blot analysis of type I collagen, α-SMA, vimentin, fibronectin and Lin28a in TGF-β-treated HK-2 cells. Cells were treated with TGF-β (5 ng/ml) for 24 h. (B) Quantification of western blot analysis results expressed as the mean ± SEM of three independent measurements. GAPDH levels were analyzed as an internal control. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with TGF-β (−).