Fig. 2.

MSCs derived hypoxic conditioned medium promotes M2 macrophage polarization. (A) The morphology of polarized BMDMs treated with hypo-CM derived from MSCs were similar to IL-4. (B, C) De-crease of M1 polarization is presented through treatment of hypo-CM derived from MSCs in vitro. Treatment of hypo-CM derived from MSCs in vitro resulted in increased M2 polarization. Control, BMDMs treated with 10% FBS medium; Nor, BMDMs treated with SF medium; Hypo, hypoxia, BMDMs treated with hypo-CM derived from MSCs. All groups were cultured at 37°C in a humidified atmosphere containing 5% CO₂. Each experiment was repeated three times. Columns, mean; bars, SE. **P <0.01 and *P <0.05, and ^##P < 0.001.