(A) Schematic protocol for transplantation of white control, HUMBLE and brown control preadipocytes into diet-induced obese (DIO) nude mice. (B) BW change over 4 weeks in DIO mice after transplantation with white control, HUMBLE or brown control preadipocytes; n = 6 mice per group. (C and D) GTT (C) and ITT (D) in DIO mice 4 weeks after transplantation with white control, HUMBLE or brown control cells; n = 6 mice per group. (E and F) Serum glucose (E) and insulin (F) concentration in DIO mice 4 weeks after transplantation with white control, HUMBLE or brown control preadipocytes; n = 6 mice per group. (G) H&E staining of BAT (upper), subcutaneous WAT (scWAT; middle), and liver (lower) from DIO mice 4 weeks after transplantation with white control, HUMBLE, or brown control preadipocytes. Data are shown as representative micrographs with 100 μm scale bars. In all charts, data are presented as means ± s.e.m.; *p < 0.05; **p < 0.01.