Table 2.
Included studies summarization.
| Reference | Type of injection | Aims | Company produced irisin | Mice type and info | Significant dose μg/ kg | Dose frequency |
|---|---|---|---|---|---|---|
| Asadi et al. [16] | ICV Intracere broventricularly | Protect against brain ischemic injury | Orb180476 Biorbyt Cambridge, UK | Male Swiss albino mice 30-35 g 42 mice | 7.5 μg/kg | Single dose after ischemia |
| Colaianni et al. [9] | Injected only (not mentioned) | Low=Increase in cortical bone and strength High=Cause browning of adipose tissue | AG-40B-0103 Adipogen | C57BL/6 male 2 months | Low dose 100 μg/kg High dose 500 μg/kg | Weekly (4 weeks) Daily (3500 μg/kg weekly) for 2 weeks |
| Zhao et al. [17] | Tail intravenous injection | Beneficial against ischemia-reperfusion injury after perforator flap grafting in rats | NM | Sprague-Dawley rats 200-250 g 48 n | 2 μg/kg | Daily The effect was significant on day 7 |
| Dameni et al. [19] | Intrathecal administration | Pain threshold and expression rate of GABAB receptors in peripheral neuropathic pain model | Orb 180477 Biorbyt Cambridge, UK | Male Wistar rats 200-250 g | 1 μg/kg | In one dose, it was affected on pain threshold but daily for 2 weeks have no effect. |
| Duan et al. [13] | Intraperitoneal injection | Could lower the glucose in diabetic mice | Gene expression in yeast P. pastoris GS115 | C57BL/6J male 3 weeks 10-12 g 54 n | 1 mg/kg | Daily for 3 weeks |
| Liu et al. [15] | Intraperitoneal injection | Low-dose irisin treatment alleviated cardiac fibrosis and left ventricular function in the diabetic mice | Gene expression in yeast P. pastoris pPICZaA plasmid Life Technology | C57BL/6J male 25-30 g | 500 μg/kg | Daily for 16 weeks |
| Colaianni et al. [10] | Intraperitoneal injection | Prevents and restores bone loss and muscle atrophy | Adipogen International (San Diego, USA) | C57BL/6 male 2 months 64 n | 100 μg/kg | Weekly for 4 weeks (once a week) |
| Zhu et al. [12] | Intraperitoneal injection | Irisin Increased the number and improved the function of endothelial progenitor cells in diabetes mellitus mice | Phoenix Pharmaceuticals | C57BL/6 mice (4 weeks old; 13-15 g | 500 μg/kg | Daily for 8 weeks |
| Majeed et al. [18] | Intraperitoneal injection | On body mass index, serum insulin, luteinizing hormone, and testosterone levels in obese female | CUSABIO | BALB/c female 6-8 week 25±5 g | 200 μg/kg | Daily for 7 days |
| Shao et al. [14] | Intraperitoneal injection | Effects of irisin on LPS-induced acute lung injury in mice | Phoenix Pharmaceuticals Inc. (Karlsruhe, Germany) | C57BL/6J male 6-8 week | 500 μg/kg | Daily for 21 days |
| Zhang et al. [11] | Intraperitoneal injection | Irisin can potentially prevent obesity and associated type 2 diabetes by stimulating expression of WAT browning-specific genes | Gene expression in yeast P. pastoris pPICZaA plasmid life | C57BL/6 mice 10 weeks old feed 35-43 g 16 n | 500 μg/kg | Daily for 14 days |
P. pastoris=Pichia pastoris