1.
RT-qPCR引物信息
Sequences and expected product lengths of the primers for RT-qPCR
| Targeted gene | Forward sequence and reverse sequence | Product length (bp) |
| Syk | F: 5'-TGGGTGGTTTTGCTTTGT-3' R: 5'-TCCTGGGAGTGGTAATGG-3' |
142 |
| c-fos | F: 5'-CCTACCCCGAGGCTGAC-3' R: 5'-CCTGCCTTCTCTGACTGCT-3' |
131 |
| Ras | F: 5'-TGGCTGGAAGTAGGAGGTG-3' R: 5'-GCAGGCAGAAGAGAAGGG-3' |
147 |
| MEK1 | F: 5'-GCGGGCAGCTAATTGAC-3' R: 5'-CCCCATGCTCCAGATGT-3' |
116 |
| MEK2 | F: 5'-CGCTCACCATCAACCCTAC-3' R: 5'-CCGCTTCCTCTGCTGTTC-3' |
128 |
| ERK-1 | F: 5'-GAACCCCACCCCATTTTC-3' R: 5'-TCCACATCCAATCACCCA-3' |
148 |
| ERK-2 | F: 5'-CGGCGGTTAGTTCTCTCTT-3' R: 5'-GACTTGTGCTGCGCTTC-3' |
109 |
| β-actin | F: 5'-CCTCACTGTCCACCTTCCA-3' R: 5'-GGGTGTAAAACGCAGCTCA-3' |
120 |