2.
反转录反应体系
Reaction system of RT-PCR
| Reagents | Volume (μL) |
| Reaction mixture form step1 | 10.0 |
| Prime script RT enzyme mix 1 | 1.0 |
| RT primer mix | 1.0 |
| 5 x prime script buffer 2 (for real time) | 4.0 |
| RNase Free dH2O | 4.0 |
| Total | 20.0 |
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