3.
Real-time PCR反应体系
Reaction system of real-time PCR
| Reagents | Volume (μL) |
| SYBR premix Ex TaqrM (x2) | 5.0 |
| Primers (2 g·mol/L) 0.2 μL | 1.0 |
| ROX reference dye (x50) 1.0 μL | 0.2 |
| RNase inhibitor | 1.0 |
| cDNA | 3.8 |
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