4.
Real-time PCR反应程序
Reaction program of Real-time PCR
| Step | Temperature (℃) | Time | Repeats |
| 1 | 50 | 2 min | 1 |
| 2 | 95 | 10 min | 1 |
| 3 | 95 | 15 s | 40 |
| 3 | 60 | 1 min | 40 |
| 4 | 95 | 15 s | 1 |
| 4 | 60 | 15 s | 1 |
| 4 | 95 | 15 s | 1 |
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