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. 2020 Nov 25;14(1):100959. doi: 10.1016/j.tranon.2020.100959

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 2 — CTCs staining results of breast cancer patients. (A) CTCs in the blood samples of the breast cancer patients were marked as CK+/DAPI+/CD45-. (B) Representative images of white cell cluster (up) staining with CK- (green)/ CD45+ (orange) /DAPI+ (nuclei, blue); CTCs cluster (down) staining with CK+ (green) /CD45- (orange) /DAPI+ (nuclei, blue), isolated by the chip. Scale bars 20 μm. (C) Typical images of different types of cells tested include CK+/PDL-1+/CD45- (red arrow), CK+/PDL-1-/CD45- (orange arrow), CK+/Vimentin-/CD45- (green arrow), CK-/Vimentin+/CD45- (white arrow), CK+/Vimentin+/CD45- (yellow arrow). Scale bars 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)