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Single PCR amplification at different annealing temperature. Lane M, D2000 DNA marker; Lane 1, 52.0°C; Lane 2, 52.9°C; Lane 3, 53.8°C; Lane 4, 54.9°C; Lane 5, 56.0°C; Lane 6, 57.0°C; Lane 7, 58.1°C; Lane 8, 59.2°C; Lane 9, 60.1°C; Lane 10, 61.0°C. (A) E. coli; (B) K. pneumoniae; (C) Salmonella; (D) S. aureus.
