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. 2020 Nov 17;11:611188. doi: 10.3389/fpls.2020.611188

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Copyright © 2020 Beihammer, Maresch, Altmann and Strasser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) LC-ESI-MS/MS analysis of the C-terminal peptide from RFP-COB1. RFP-COB1 was expressed in A. thaliana seedlings, captured by binding to RFP-Trap resin and subjected to PI-PLC and trypsin digestion. (B) RFP-COB1 is found at the plasma membrane. RFP-COB1 was transiently expressed in Nicotiana benthamiana leaf epidermal cells and analyzed by confocal microscopy. (C) LC-ESI-MS analysis of β-galactosidase digested GPI-anchor derived from A. thaliana expressed RFP-COB1. (D) The transferred GPI backbone is further modified by different enzymes, including inositol deacylation, the remodeling of the lipid portion (depicted by the change in color from black to red in the illustration) and the EtNP removal, which is likely required for recognition by p24 cargo receptor proteins and efficient ER exit. In the proposed model, the plant-specific side chain modification is transferred in the Golgi by an unknown β-galactosyltransferase (GALT).