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. 2020 Nov 30;11:6119. doi: 10.1038/s41467-020-19973-6

Fig. 3. CCL7 deficiency impairs cDC1 infiltration to tumor-burdened lung.

Fig. 3

a Gating strategy of single-cell suspensions from lungs of KP mice that were intranasally injected with Ad-Cre (1 × 106 pfu/mouse) for 10 weeks. b Cell numbers of tumor-burdened lungs of KP (n = 15) and KP7 (n = 15) mice intranasally injected with Ad-Cre (1 × 106 pfu/mouse) for 10 weeks. c Flow cytometry analysis of single-cell suspensions of tumor-burdened lungs (left flow chart) and percentages and numbers of CD11c+CD11b− cells in tumor-burdened lungs (left graphs) of KP and KP7 mice treated as in b. d Percentages and numbers of CD11c+CD11b−CD103+H-2Kb+ DCs in tumor-burdened lungs of KP and KP7 mice treated as in b. e Flow cytometry analysis of CD11c+CD11b− in d stained with CD103, H-2Kb, CD86, XCR1, CD24, CD64, CCR1, CCR2, and CCR3. f IHC (images) and intensity or IOD (graphs) analysis of CD11c, CD103, XCR1, and CCL7 in tumor sections of KP (n = 5) or KP7 (n = 5) mice injected with Ad-Cre (1 × 106) for 10 weeks. Two-tailed student’s t-test (b–d, f). n.s., not significant. Graphs show mean ± SEM (b–d, f). Scale bars, 50 μm. Data are combined results of four (b–e) or two (f) independent experiments. Source data are provided as a source data file.