Fig. 5. Depletion of CD11c⁺ DCs promotes tumorigenesis of KP mice.

a A scheme of adoptive transfer of CD11c-DTR bone marrow cells and DT-mediated depletion of CD11c⁺ DC depletion in KP or KP7 mice after tumor induction. b Flow cytometry analysis of single-cell suspensions of tumor-burdened lungs from KP (n = 4 and 5 for PBS and DT, respectively) and KP7 (n = 4 and 5 for PBS and DT, respectively) mice treated as in a stained with CD11b, CD11c, CD103, and H-2K^b (left flow charts). The numbers of CD11c⁺CD11b⁻CD103⁺H-2K^b+ DCs were calculated (right graphs). c, d HE-stained lungs (c) and tumor burden analysis of the lungs (d) from KP (n = 4 and 11 for PBS and DT, respectively) and KP7 (n = 5 and 9 for PBS and DT, respectively) mice treated as in a. e Flow cytometry analysis of CD3⁺ cells in bronchial dLNs of KP (n = 4 and 5 for PBS and DT, respectively) and KP7 (n = 4 and 5 for PBS and DT, respectively) mice treated as in a stained with CD4, CD8, and IFNγ (left flow charts). The numbers of total cells and CD8⁺IFNγ⁺ cells in dLNs were calculated (right graphs). f, g IHC (f) and intensities (g) analysis of CD11c and CD8 in tumors from KP (n = 4 and 11 for PBS and DT, respectively) or KP7 (n = 5 and 9 for PBS and DT, respectively) mice treated as in a. Two-tailed student’s t-test (b, d, e, g). n.s., not significant. Graphs show mean  ± SEM (b, d, e, g). Scale bars, 50 μm. Data are representative of two independent experiments. Source data are provided as a source data file.