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. 2020 Nov 30;11:6118. doi: 10.1038/s41467-020-19961-w

Fig. 4. Loss of HUWE1 results in increased RAD51 levels and partial restoration of homologous recombination in BRCA2-deficient cells.

Fig. 4

a Knockout of HUWE1 rescues the olaparib sensitivity of BRCA2-depleted HeLa cells in cellular viability assays. The graph presents the average of three experiments performed after 72 h of treatment, with standard deviations shown as error bars. b Quantitative PCR assay showing that HUWE1 depletion increases RAD51 mRNA levels. Cells were treated with DMSO or 10 μM olaparib for 24 h, as indicated. Data shown are the average of three experiments, normalized to the siControl condition, with standard deviations shown as error bars. Statistical significance is indicated by asterisks (t-test, two-tailed, unequal variance). c Western blot of whole-cell extracts showing that HUWE1 knockdown increases RAD51 protein levels. Cells were treated with DMSO or 10 μM olaparib for 24 h, as indicated. d HUWE1 depletion partially rescues the homologous recombination defect caused by BRCA2 knockdown as shown using a DR-GFP assay. The averages of four experiments are shown, with standard deviations as error bars. Statistical significance is indicated by asterisks (t-test, two-tailed, unequal variance). Source data are provided as a Source Data file.