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. 2020 Nov 30;11:6118. doi: 10.1038/s41467-020-19961-w

Fig. 7. Loss of KAT5 expression causes resistance to cisplatin in BRCA2-deficient cells.

Fig. 7

a In clonogenic survival assays, depletion of KAT5 in BRCA2-knockout HeLa cells led to increased colony formation after cisplatin treatment (24 h). The averages of three experiments are shown, with standard deviations as error bars. b DNA fiber combing assay showing that KAT5 depletion partially suppresses the HU-induced nascent DNA degradation in BRCA2-deficient cells. The ratio of CldU to IdU tract lengths is presented, with the median values marked on the graph. At least 150 replication tracts were quantified for each sample. Asterisks indicate statistical significance (Mann–Whitney test, two-sided). c Bioinformatic analysis of the OV-TCGA dataset shows that patients with tumors that have low KAT5 expression trend towards poorer survival in BRCA2 mutant (left) but not BRCA2 wildtype (right) tumors. The difference in BRCA2 mutant samples is not significant (p = 0.28) likely because of the small number (n = 20) of BRCA2-mutant samples in this dataset. Source data are provided as a Source Data file.