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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Adv Biosyst. 2020 Sep 13;4(11):e2000129. doi: 10.1002/adbi.202000129

Figure 4.

Figure 4.

(A) Timeline of ChiPSC12 cells in statically soft, statically stiff, and dynamically stiffened hydrogels on day 7 post-encapsulation. For the dynamically stiffened condition, PEG-Tz (0.2 wt%) was added to the media for 24 hours on D3–D4. (B) Live/Dead staining and imaging of ChiPSC12 cell line on day 7 post-encapsulation. (C) Representative slice of F-actin and nuclear staining and imaging on day 7 post-encapsulation. (D) Distribution of aggregate diameters with or without Tz-NB stiffening. The diameters of the aggregates for non-dynamic soft, non-dynamic stiff and dynamically stiffened conditions were 95.8 ± 3.13 μm (N = 140), 144.1 ± 10.0 μm (N = 35), and 80.1 ± 1.9 μm (N = 152), respectively. Aggregate diameters were analyzed using a Kolmogorov-Smirnov test of the cumulative frequency distributions.. Significance between soft and stiff (p < 0.001),stiffened and stiff (p < 0.001), and soft and stiffened (p < 0.01) was determined.