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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Neurochem Int. 2020 Oct 22;141:104888. doi: 10.1016/j.neuint.2020.104888

Figure 4: Comparison of antioxidant levels between wild-type (+/+) and system xc- null (sut/sut) female mice in striatum, hippocampus, and cortex.

Figure 4:

Bilateral striatal, hippocampal, or cortical tissue was pooled from one mouse per sample and the protein extracted was separated under reducing conditions via 8% (catalase), 11% (SOD2), or 15% (SOD1) SDS-PAGE. Representative blot for detection of SOD1, SOD2, and catalase protein in striatum (A), hippocampus (B), and cortex (C). Protein quantification was performed by normalizing to each samples’ representative total protein to correct for differences in loading followed by baseline correction to the protein +/+ levels. Values are graphed as Tukey’s box and whisker plots with the box representing the 2nd and 3rd quartiles and whiskers representing points within 1.5 times the interquartile distance. There are no between-group differences calculated using a one-sided t-test with Welch’s correction. (A) Striatum: SOD1 (F1,6 = 36.86; p = 0.066), SOD2 (F1,6 = 1.022; p = 0.190) and catalase (F1,6 = 178.9; p = 0.087). (B) Hippocampus: SOD1 (F1,5 = 2.468; p = 0.383), SOD2 (F1,5 = 1.318; p = 0.364) and catalase (F1,6 = 1.922; p = 0.230). (C) Cortex: SOD1 (F1,6 = 4.702; p = 0.231), SOD2 (F1,6 = 2.655; p = 0.116) and catalase (F1,6 = 2.045; p = 0.448).