Figure 5.

Human Neutrophils in Peripheral Blood of Recipient Mice Reconstituted with ELANE-Corrected HSPCs

(A) Experimental scheme of ELANE^L172P correction in SCN-HSPCs and transplantation of these cells into humanized NOG-EXL mice. Human cells were monitored in the peripheral blood of transplanted mice every 4 weeks. (B) FACS data showing the percentage of human CD45⁺ cells and standard saline citrate (SSC)^hiCD66b⁺ neutrophils in the peripheral blood of recipient animals reconstituted with ELANE-repaired (orange) or non-repaired (ELANE-L172P, black) HSPCs at 8 weeks post-transplantation. (C) Percentages of human CD45⁺ cells (left) and human SSC^hiCD66b⁺ neutrophils (right) in the peripheral blood of reconstituted mice at the indicated time points after transplantation. Data represent mean ± SD for three mice. See also Figures S7 and S8.