Figure 4.

Combination of In Situ Acute 3WJ-L12 and Subacute iNSC Transplantation Promotes Functional Recovery

(A) Generic 3WJ scheme depicting the three constituent RNA strands, variable siRNA and 3WJ core moieties (black circles indicate a wobble base pair). Target sequences of the variable siRNA moiety (N) are depicted for the anti-Lcn2 (3WJ-L12) and the non-targeting 3WJ-NT. (B) Schematic representation of experimental design for acute injection of 3WJ-L12 into the lesion epicenter followed by subacute transplantation of fGFP-iNSCs. (C) Basso Mouse Scale (BMS) score evaluation of motor function recovery. Data are mean values (±SEM) from n $\ge$ 7 mice per group. ∗p < 0.05; ∗∗p < 0.01, and ∗∗∗p < 0.001, 3WJ-L12-only versus 3WJ-NT/iNSCs. #p < 0.05, 3WJ-L12/iNSCs versus 3WJ-NT/iNSCs. (D) Assessment of mechanical allodynia by von Frey test. Data are mean values (±SEM) from n $\ge$7 mice per group. dpi, days post injury.