Skip to main content
. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Matrix Biol. 2020 Jun 11;93:25–42. doi: 10.1016/j.matbio.2020.06.001

Figure 3.

Figure 3.

Heparanase is a convenient marker of the tumor microenvironment. A. Immunostaining. Five-micron sections of tumor xenografts produced by PANC02 (upper panels) and B16 melanoma (lower panels) cells in WT and Hpa-tg (Tg) mice were subjected to immunostaining employing anti-heparanase antibody. Note, abundant heparanase-positive stromal cells in Hpa-tg tumors produced by Panc02 but not B16 melanoma cells. B. Heparanase activity. Heparanase enzymatic activity was evaluated in corresponding tumor extracts (Panc02, upper panel; B16 melanoma, lower panel). Note that the recruitment of stromal cells correlates with increased heparanase activity in tumors produced by Panc02 cells.