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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Matrix Biol. 2020 Jun 11;93:25–42. doi: 10.1016/j.matbio.2020.06.001

Figure 5. Overexpression of host heparanase accelerates tumor metastasis.

Figure 5.

A. Experimental metastasis. LLC2 cells (1×106) suspended in 100 μl PBS were injected into the tail vein of Hpa-tg and WT C57BL mice and the lungs were dissected on day 17 post-injection. Lung colonization by tumor foci was visualized after fixation of the tissue in Bouin’s solution (left panel). Lungs were excised from Hpa-tg and WT mice and their weights measured (right panel). B. Spontaneous metastasis. LLC cells (3.5×105) were injected S.C to WT (cont.) and Hpa-tg (TG) mice; tumors were removed after 18 days when approaching a similar tumor volume (750 mm3), and survival of the mice was monitored (left panel). Lungs were excised from Hpa-tg and WT mice and their weights measured (right panel).