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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Matrix Biol. 2020 Jun 11;93:25–42. doi: 10.1016/j.matbio.2020.06.001

Figure 6.

Figure 6.

Host heparanase facilitates pancreatic tumor metastasis associating with reduced survival. A,B. Inoculation (i.v) of Panc02 mouse pancreatic cancer cells to WT and Hpa-tg mice. A. Representative MRI images of WT and Hpa-tg mice on day 40 following Panc02 cell injection. Note, many tumor lesions in Hpa-tg mice (arrows) vs. none in WT mice. B. Survival of WT and Hpa-tg mice following Panc02 cell inoculation. Note, reduced survival of Hpa-tg mice. C,D. BM transplantation. WT mice were lethally irradiated and their BM was replaced with BM cells (5×106) collected from WT (WT-WT) or Hpa-tg (Tg-WT) tibias. Panc02 cells were injected i.v six weeks thereafter and the survival rate was followed (C). Note a significant decrease in the survival rate of mice that have high heparanase levels in BM-derived cells. D. Immunostaining. Upon termination, tumors were excised and subjected to immunostaining applying anti-F4/80 antibody, a marker for mouse macrophages. Note, high abundance of macrophages in tumors developed in Hpa-tg mice.