Figure 2.

Measurement of Cardiac Pathological and Structural Alterations after GW4869 Treatment

(A) Heart-to-body weight (HW/BW) ratios were measured on day 21. (B) Cardiac tissue H&E staining was performed (scale bars, 100 μm). (C). The H&E images were analyzed based on histological grades. (D) Serum cardiac troponin I was determined by ELISA. (E and F) CD4⁺ cell infiltration in the cardiac sections was analyzed by immunohistochemistry (scale bars, 100 μm). (G) Cardiac M-mode echocardiographic images are displayed. (H–K) Left ventricular ejection fractions (EFs; H), left ventricular end-diastolic dimension (LVEDD; I), left ventricular end-systolic dimension (LVESD; J), and LV fractional shortening (FS; K) were measured in respective groups. Data are presented as the means ± SEM (n = 6); ∗p < 0.05.