TABLE 5.

Protein levels of nitric oxide synthases, growth factors, mitochondrial biogenesis factors, and apoptosis mediating factors in LV myocardium of normal dogs (NL), untreated cardiorenal syndrome (CRS) control dogs (CRS-CON) and CRS dogs treated with Sac/Val (CRS+Sac/Val).

	Normal (n=7)	CRS-CON (n=7)	CRS+Sac/Val (n=7)
eNOS (du)	1.81 ± 0.20	0.47 ± 0.04*	1.35 ± 0.11†
iNOS (du)	0.37 ± 0.04	1.70 ± 0.02*	0.62 ± 0.07*†
PGC-1α (du)	2.12 ± 0.29	0.47 ± 0.05*	1.08 ± 0.14*†
Active Caspase-3 or p17 du)	1.27 ± 0.15	3.87 ± 0.13*	2.78 ± 0.14*†
Cytosolic Cytochrome c (du)	0.58 ± 0.14	4.35 ± 0.23*	1.78 ± 0.18*†

Sac/Val=sacubitril/valsartan; eNOS=endothelial nitric oxide synthase; iNOS=inducible nitric oxide synthase; mitochondrial; PGC-1α= peroxisome proliferator-activated receptor coactivator-1α; du=densitometric units.

^*=p<0.05 vs. Normal;

^†=p<0.05 vs. CRS-CON.