Skip to main content
. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Circulation. 2020 Sep 16;142(22):2138–2154. doi: 10.1161/CIRCULATIONAHA.119.044805

Figure 3. SRF S103 phosphorylation induces transcriptional activity at select enhancers in adult rat ventricular myocytes.

Figure 3.

A, Volcano plot showing the binding sites for Ser103 phosphorylated-SRF increased (Group 1), unchanged (Group 2), or decreased (Group 3) by false discovery rate < 0.05 in ChIP-seq using phospho-Ser103-specific antibody for myocytes treated for 1 hour +/− phenylephrine (PE). B, Heatmaps showing phospho-Ser103-specific antibody ChIP-seq signals over individual sites in Groups 1-3 as defined in A. C, Violin and box-and-whisker plots showing normalized SRF antibody ChIP-seq tags for each Group. D, Average enrichment profile (sites with significant counts only) for H3K27Ac ChIP-seq signals relative to the center of H3K27Ac-marked enhancers within the indicated Groups. E, Plots showing normalized Pol II antibody ChIP-seq tags for H3K27Ac-marked enhancers within the indicated Groups. F, Same as E for myocytes expressing Flag-tagged SRF WT, S103A, and S103D protein. For all violin blots, center lines show the medians (values in parentheses below), box limits indicate the 25th and 75th percentiles, and whiskers extend 1.5× the interquartile range from the 25th and 75th percentiles.