KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-APP [Y188] | abcam | AB_2289606 |
| Rabbit anti-APP-CTF | Sigma-Aldrich | AB_258409 |
| Rabbit anti-APLP1 | D. Walsh's Lab | N/A |
| Rabbit anti-APLP1-C-term [643–653] | Millipore | AB_565288 |
| Rabbit anti-APLP2 | D. Walsh's Lab | N/A |
| Rabbit anti-APLP2-C-term [752–763] | Millipore | AB_211446 |
| Mouse anti-NeuN | Millipore | AB_2298772 |
| Mouse anti-GAD67 | Millipore | AB_2278725 |
| Rabbit anti-Cleaved Caspase3 (Asp175) | Cell signaling | AB_2341188 |
| Mouse anti-MAP2 | abcam | AB_776174 |
| Rabbit anti-PSD95 | Cell signaling | AB_1264242 |
| Rabbit anti-Synaptophysin | Cell signaling | AB_10698743 |
| Rabbit anti-SNAP25 | abcam | AB_10888111 |
| Mouse anti-GFAP | Sigma-Aldrich | AB_1840893 |
| Mouse anti-β-actin | Sigma-Aldrich | AB_476692 |
| Mouse anti-β-actin | Cell signaling | AB_2242334 |
| Rabbit anti-β-actin | Cell signaling | AB_330288 |
| Goat Biotynylated anti-Rabbit IgG (H&L) | Vector Laboratories | AB_2313606 |
| Goat Biotynylated anti-Mouse IgG (H&L) | Vector Laboratories | AB_2336171 |
| Goat anti-Rabbit IRdye680 Secondary Antibody | LI-COR Biosciences | AB_10956166 |
| Goat anti-Mouse IRdye680 Secondary Antibody | LI-COR Biosciences | AB_2651128 |
| Goat anti-Rabbit IRdye800 Secondary Antibody | LI-COR Biosciences | AB_2651127 |
| Goat anti-Mouse IRdye800 Secondary Antibody | LI-COR Biosciences | AB_2687825 |
| Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | AB_2576217 |
| Alexa Fluor® 555 Goat Anti-Mouse IgG (H+L) | Thermo Fisher Scientific | AB_141780 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| TRIzol | Sigma-Aldrich | Cat # T9424 |
| Vectastain Elite ABC reagent | Vector laboratories | AB_2336827 |
| RIPA buffer | Thermo Fisher Scientific | Cat # 89900 |
| Protease Inhibitor Cocktail | Sigma-Aldrich | Cat # P8340 |
| Phosphatase Inhibitor Cocktail | Sigma-Aldrich | Cat # P2850 |
| Procaine hydrochlorid | Sigma-Aldrich | Cat # P9879 |
| Heparin | Sigma-Aldrich | Cat # H4784 |
| Normal Goat Serum Blocking Solution | Vector laboratories | Cat # S-1000 |
| Formaldehyde/Glutaraldehyde | Electron Microscopy Science | Cat # 15949 |
| Nitrocellulose Membrane | Thermo Fisher Scientific | Cat # 88018 |
| DAB Peroxidase Substrate Kit | Vector laboratories | AB_2336827 |
| VECTASHIELD Mounting Medium | Vector laboratories | Cat # H-1000 |
| In Situ Cell Death Detection Kit | Roche | Cat # 11684795910 |
| Cresyl violate (acetate) | Sigma-Aldrich | Cat # C5042 |
| Sodium chloride | Sigma-Aldrich | Cat # S5886 |
| Potassium chloride | Sigma-Aldrich | Cat # P9541 |
| Sucrose | Sigma-Aldrich | Cat # S7903 |
| D-(+)-Glucose | Sigma-Aldrich | Cat # G5767 |
| Sodium phosphate monobasic | Sigma-Aldrich | Cat # S5011 |
| Sodium bicarbonate | Sigma-Aldrich | Cat # S5761 |
| Sodium pyruvate | Sigma-Aldrich | Cat # P5280 |
| (+)-Sodium L-ascorbate | Sigma-Aldrich | Cat # A4034 |
| Calcium chloride dihydrate | Sigma-Aldrich | Cat # 223506 |
| Magnesium chloride hexahydrate | Sigma-Aldrich | Cat # M9272 |
| HEPES | Sigma-Aldrich | Cat # H3375 |
| EGTA | Boston Bio Products | Cat # BM-151 |
| Adenosine 5′-triphosphate magnesium salt | Sigma-Aldrich | Cat # A9187 |
| Guanosine-13C10 5′-triphosphate sodium salt | Sigma-Aldrich | Cat # G8877 |
| Potassium D-gluconate | Sigma-Aldrich | Cat # G4500 |
| Phosphocreatine disodium salt hydrate | Sigma-Aldrich | Cat # P7936 |
| Picrotoxin | Tocris | Cat # 1128 |
| APV | Tocris | Cat # 0106 |
| NBQX disodium salt | Tocris | Cat # 1044 |
| Tetrodotoxin | Tocris | Cat # 1069 |
| XE991 dihydrochloride | Tocris | Cat # 2000 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Raw and analyzed data | This paper; and Mendeley Data | http://dx.doi.org/10.17632/vs48zct2xp.1 |
| Experimental Models: Cell Lines | ||
| Mouse: MKV6.5 ES cell | R. Jaenisch's Lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6129SF1/J | The Jackson Laboratory | IMSR_JAX:101043 |
| Mouse: Camk2a-Cre transgenic | Yu et al., 2001 | N/A |
| Mouse: B6.Cg-Tg(ACTFLPe)9205Dym/J | Rodriguez et al., 2000 | IMSR_JAX:005703 |
| Mouse: fAPP/fAPP | This paper | N/A |
| Mouse: fAPLP1/fAPLP1 | This paper | N/A |
| Mouse: fAPLP2/fAPLP2 | This paper | N/A |
| Oligonucleotides | ||
| See Table S1 for The List of Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| Image-Studio | Li-Cor | SCR_013715 |
| Fiji (ImageJ) | NIH | SCR_002285 |
| PRISM 8 | GraphPad | SCR_005375 |
| Bioquant Life Science | Bioquant Life Science | SCR_016423 |
| Accuscan Versamax | AccuScan Instruments | v.1.05 |
| HVS Image 2020 Plus tracking system | HVS Image | v.5/2003 |
| FV10-ASW | Olympus | SCR_014215 |
| Clampfit | Molecular device | v.10.3 |
| Igor Pro | Wave-Metrics | SCR_000325 |
| Other | ||