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. Author manuscript; available in PMC: 2021 May 23.
Published in final edited form as: Nat Methods. 2020 Nov 23;17(12):1191–1199. doi: 10.1038/s41592-020-01000-7

Extended Data Fig. 1. Assessment of CpG and GpC dual methylation calling.

Extended Data Fig. 1

The ability of nanopore sequencing to distinguish cytosine methylation at CpG and GpC contexts is shown by (a) examining current level shifts depending on the placement of the methylation on a 6-mer (n = 256 unique 6mers for each group). Data are presented as median values, interquartile range (IQR), and 1.5X IQR. The performance of the methylation caller was validated by (b) measuring methylation frequencies for calling methylation in samples treated by methyltransferases.