Figure 5. Ly6G^Mid granulocytes are retained in the lungs at least as long as monocytes/macrophages.

a Schematic of in vivo pulmonary cell half-life assay (top) and representative histogram overlays (bottom) of Bl6 WT pulmonary CD11b^High Ly6G^Pos cells or CD11b^High Ly6G^Neg monocytes/macs left untreated (red) or stained with CellTrace™ Violet via tracheal aspiration and measured at 1 hour (gold) or 48 hours (blue) post-labeling. b Mean half-life of total or c infected YFP^Pos (red) vs uninfected YFP^Neg (black) Ly6G^Neg monocytes/macrophages, Ly6G^Mid granulocytes, or Ly6G^High neutrophils in the lungs of Bl6 WT or Nos2^−/− mice. Error bars represent standard deviation between half-life values calculated from at least 3 separate pairs of biological replicates (mice). N = 3 mice per genotype per time-point post-treatment. Statistical values based on two-way ANOVA with Tukey’s Multiple Comparison analysis.