Fig. 11: Apoptotic effects of IR, GC4419, and combination in lung cancer and normal immortalized cells.
Lung cancer cells A549 and H1299 or normal immortalized cells Beas 2b were either left untreated or treated with 2 Gy (IR), 10 μM GC4419 (GC;), or combination then maintained for 48 hr in a humidified atmosphere of 95% air: 5% CO2 at 37 0C . Cells were washed, trypsinized, and DNA fragmentation (A) and Caspase-3 activity (B) were performed. Results are presented as mean ± SEM of three independent experiments. *significant from control (p < 0.05), ** significant from control (p<0.001), # significant from IR (p<0.001).