Extended Data Table 1.
Cryo-EM data collection, refinement and validation statistics
| mcGAS/nucleosomex (reconstituted. 1:1) (EMDB-22046) (PDB 6X59) |
mcGAS/nucleosome (reconstituted, 2:1) (EMDB-22206) (PDB 6XJD) |
mcGAS/nucleosome (HEK 293T, 1:1) (EMDB-22047) (PDB 6X5A) |
|
|---|---|---|---|
| Data collection and processing | |||
| Magnification | 130,000 | 130,000 | 130,000 |
| Voltage (kV) | 300 | 300 | 300 |
| Electron exposure (e–/Å2) | 42 | 42 | 42 |
| Defocus range (μm) | −1.8 ~ −0.7 | −1.8 ~ −0.7 | −2.0 ~ −0.8 |
| Pixel size (Å) | 1.07 | 1.07 | 1.07 |
| Symmetry imposed | C1 | C1 | C1 |
| Initial particle images (no.) | 2,202,680 | 2,202,680 | 578,302 |
| Final particle images (no.) | 165,092 | 9,454 | 23,463 |
| Map resolution (Å) | 2.96 | 6.79 | 4.36 |
| FSC threshold 0.143 | |||
| Map resolution range (\) | 6.0 ~ 2.8 | 10.7 ~ 4.1 | 9.1 ~ 3.9 |
| Refinement | |||
| Initial model used (PDB code) | 3AFA 4K8V | 3AFA 4K8V | 3AFA 4K8V |
| Model resolution (Å) | 3.00 | 7.16 | 4.54 |
| FSC threshold 0.5 | |||
| Model resolution range (Å) | 32.5 ~ 2.8 | 39.8 ~ 6.8 | 35.3 ~ 4.4 |
| Map sharpening B factor (Å2) | −15 | −48 | N/A |
| Model composition | |||
| Non-hydrogen atoms | 14,963 | 17,957 | 14,963 |
| Protein residues | 1,123 | 1,485 | 1,123 |
| Nucleotide | 290 | 290 | 290 |
| Ligands | 1 | 2 | 1 |
| B factors (Å2) | |||
| Protein | 83.69 | 104.67 | 405.53 |
| Nucleotide | 74.19 | 74.19 | 278.45 |
| Ligand | 194.81 | 194.81 | 997.41 |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.006 | 0.008 | 0.007 |
| Bond angles (°) | 0.834 | 1.533 | 0.887 |
| Validation | |||
| MolProbity score | 2.64 | 2.73 | 2.23 |
| Clashscore | 10.75 | 10.82 | 13.71 |
| Poor rotamers (%) | 7.57 | 8.16 | 1.66 |
| Ramachandran plot | |||
| Favored (%) | 93.67 | 91.95 | 93.67 |
| Allowed (%) | 6.15 | 8.05 | 6.15 |
| Disallowed (%) | 0.18 | 0 | 0.18 |