Table 2.

Diet formulation and calculated nutrient composition for laying period (18–95 wk).¹

Ingredients, %	Peaking2	Layer 2	Layer 3	Layer 4	Layer 5
Corn	53.61	62.99	61.54	64.18	62.57
Soybean meal -48%	28.10	21.35	19.99	17.77	17.90
Soybean oil	3.75	2.90	3.00	2.87	3.21
Limestone	7.44	6.89	6.87	7.13	7.33
Oyster shell	3.19	2.95	2.94	3.06	3.14
Defluorinated phosphate	2.55	2.09	1.89	1.52	1.47
Common salt	0.30	0.30	0.30	0.30	0.30
L-Lysine HCl	0.46	0.09	0.04	0.05	0.04
DL-Methionine	0.33	0.22	0.17	0.14	0.14
Threonine	0.11	0.06	0.03	0.03	0.02
Vitamin premix3	0.05	0.05	0.05	0.05	0.05
Mineral premix4	0.06	0.06	0.06	0.06	0.06
Sand	0.05	0.05	3.11	2.84	3.78
ME (kcal/kg)	2,840	2,900	2,820	2,840	2,820
CP%	19.05	16.15	15.27	14.42	14.32
Ca%	4.94	4.48	4.40	4.42	4.51
Available P (%)	0.58	0.49	0.45	0.38	0.37

Abbreviation: 25OHD, 25-hydroxyvitamin D₃.

¹Treatments were added as a form of vitamin premix in the diet: D treatment: vitamin D₃ at 2,760 IU/kg; DD treatment: vitamin D₃ at 5,220 IU/kg; 25D treatment: vitamin D₃ at 2,760 IU/kg plus 25OHD at 2,760 IU (69 μg)/kg.

²Peaking (18–38 wk), layer 2 (39–48 wk), layer 3 (49–60 wk), layer 4 (61–75 wk), and layer 5 (76–95 wk).

³Supplied per kilogram of diet: vitamin A, 9,900 IU; vitamin E, 22.10 IU; vitamin B₁₂, 0.02 mg; biotin, 0.06 mg; menadione, 3.3 mg; thiamine, 2.20 mg; riboflavin, 6.60 mg; pantothenic acid, 11.00 mg; vitamin B₆, 4.40 mg; niacin, 33.00 mg; folic acid, 0.90 mg; choline, 191.36 mg.

⁴Supplied per kilogram of diet: Mn, 80.4 mg; Zn, 64.2 mg; Mg, 16.08 mg; Fe, 15.78 mg; Cu, 2.4 mg; I, 0.6 mg; Se, 0.24 mg.