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. 2020 Jun 17;13(6):621–631. doi: 10.1007/s12195-020-00623-2

Figure 5.

Figure 5

SIRT1 overexpression suppress fluid shear stress induced mechanical damage. (a) Detection of SIRT1 Overexpression-GFP efficiency in transient transfected cortical neurons by fluorescence assay. (b) Detection of SIRT1 overexpression efficiency in transient transfected cortical neurons by RT-PCR. (c) Detection of SIRT1 overexpression efficiency in transient transfected cortical neurons by Western blotting. (d) Photomicrographs and (e) histograms show that as shear stress time increased (from 4, 8, to 12 h) TUNEL positive cells in SIRT1 overexpressed cortical neurons were less than control group by TUNEL assay. *p < 0.05, **p < 0.01 and ***p < 0.001 were considered to be statistically significant.