Table 2.
Step | Condition |
---|---|
1 | 94 °C, 5 min |
2 | 94 °C, 30 s |
3 | 58 °C, 30 s |
4 | 72 °C, 10 s |
5 | Back to step 2 for 35 cycles |
6 | 72 °C, 10 min |
Step | Condition |
---|---|
1 | 94 °C, 5 min |
2 | 94 °C, 30 s |
3 | 58 °C, 30 s |
4 | 72 °C, 10 s |
5 | Back to step 2 for 35 cycles |
6 | 72 °C, 10 min |