Table 2.
The primer sequence and parameters.
| Genes | NCBI ID no. | Primer sequence (5’→3′) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| β-actin | GenBank: L08165.1 | GTGTGATGGTTGGTATGGGC | 225 | 59 |
| CTCTCTTGGCTTTGGGGTTC | ||||
| CPT-I | GenBank: AY675193.1 | GAAGACGGAACACTGCAAAGG | 223 | 60 |
| GGGCAAGTTGAATGAAGGCA | ||||
| ACC | NCBI Reference Sequence: NM_205505.1 | GCTGGGTTGAGCGACTAATG | 173 | 59 |
| GGGAAACTGGCAAAGGACTG | ||||
| SREBP-1c | GenBank: AY029224.1 | TGGCTGAGATCTATGTGGCG | 173 | 59 |
| GAAGAAGCGGTGACCCAATG | ||||
| SREBP-2 | GenBank: AJ414379.1 | AAGCAACCAGACCCACCTAA | 230 | 59 |
| GCCAGCTTCAGAACCATGTT | ||||
| HMGCR | GenBank: AB109635.1 | GCCGTATTCTTGCAGATGGG | 230 | 60 |
| CATTCCCATTGCATCCCCTG | ||||
| LXR-α | GenBank: AJ507202.1 | ATAGCAAGCCCTTCCTGTGT | 248 | 59 |
| ACGTTGTAGTGGAAGCCTGA | ||||
| CYP7α1 | NCBI Reference Sequence: NM_001001753.1 | TGACCCAGCAGAAGGAAACA | 159 | 59 |
| ACCCAGGTGTTAGGCTGAAA |
Abbreviations: ACC, acetyl CoA carboxylase; CPT-I, carnitine palmitoyltransferase I; CYP7α1, cytochrome P450 family 7 subfamily A member 1; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; LXR-α, liver X receptor α (LXR-α); SREBP-1c, sterol regulatory element-binding protein 1c; SREBP-2, sterol regulatory element-binding protein 2.