Table 2.

Details of treatment arrangements in studies 1 and 2.¹

Treatment	Target DEP supplementation, U/tonne of feed	Eimeria infection
Study 1
UCON	0	None
ICON	0	E. acervulina
I-143	143	E. acervulina
I-287	287	E. acervulina
Study 2
UCON	0	None
ICON	0	E. tenella
I-165	165	E. tenella
I-287	287	E. tenella

Abbreviations: DEP, dried egg product; ELISA, enzyme-linked immunosorbent assay; I-143, infected birds receiving 143 U/tonne of DEP; I-165, infected birds receiving 165 U/tonne of DEP; I-287, infected birds receiving 287 U/tonne of DEP; ICON, infected control; UCON, uninfected control.

¹Both study 1 and 2 were 26-day long, starting at approximately 2 d after hatch, and each treatment included 15 replicate cages of 12 birds (4 treatments × 15 replicates × 12 birds). All birds in the infected group received 1 mL of suspension of either 200,000 E. acervulina (study 1) or 80,000 E. tenella (study 2) sporulated oocysts in water at study day 12. All control birds were sham inoculated with 1 mL of tap water. A common basal starter diet (Table 1) was topped off with the DEP to prepare experimental diets. All diet groups were analyzed for anti–IL-10 activity after diet preparation using ELISA (Sand et al., 2016). The UCON and ICON diet groups were analyzed to contain zero activity in both studies.