. 2020 Sep 12;99(12):6493–6502. doi: 10.1016/j.psj.2020.09.012

Table 6.

Peripheral blood T-cell immunophenotypes of birds 6 d post infection with E. tenella and supplemented with the dried egg product containing anti–IL-10 antibody via feed (study 2).¹

Outcome²	Treatment				Pooled SEM	Model, P-value
Outcome²	UCON	ICON	I-165	I-287	Pooled SEM	Model, P-value
Total T cells, % (CD3⁺)	6.01^b	6.68^b	13.58^b	26.67^a	4.278	0.002
Helper T cell, % (CD3⁺CD4⁺)	2.19	1.93	1.89	4.37	1.223	0.304
Cytotoxic T cell, % (CD3⁺CD8⁺)	0.47	1.29	1.32	1.74	0.467	0.175
Memory T cell, % (CD3⁺CD4⁺CD8⁺)	0.03	0.04	0.08	0.05	0.024	0.355

^{a, b}Means lacking a common superscript letter within a row differ (P < 0.05).

Abbreviations: DEP, dried egg product; I-165, infected birds receiving 165 U/tonne of DEP; I-287, infected birds receiving 287 U/tonne of DEP; ICON, infected control; UCON, uninfected control.

The DEP was supplemented at a dose of 165 or 287 U/tonne. All birds in the infected groups received an oral inoculum containing 80,000 sporulated oocysts of E. tenella. Least square means derived from samples derived from 1 bird in each of 7 replicate battery cages (n = 7) at each independent collection time point.

Total T cells were expressed as a percentage of total cells isolated by extraction of peripheral blood mononuclear cell extraction and expressing the CD3 cell surface marker. Helper, cytotoxic, and memory T-cell populations are expressed as a percentage of total CD3⁺ T cells.