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. 2020 Dec 1;7:204. doi: 10.1038/s41438-020-00433-7

Fig. 3. MdSND1 enhanced the tolerance of transgenic apple plants to salt and osmotic stress.

Fig. 3

A MdSND1-overexpressing and MdSND1-RNAi apple plants showed different phenotypes under salt and osmotic stresses. Two hundred millimolar NaCl or 300 mM mannitol was added to the medium when the nontransgenic GL-3 apple plants and transgenic apple were twenty days old. B Detection of ROS levels in apple leaves of plants under salt and mannitol treatment for 10 days according to nitro blue tetrazolium (NBT) staining. The darker the blue precipitate is, the higher the ROS level. C Detection of ROS levels in apple leaves of plants under salt and mannitol treatment for 10 days according to 3,3’-diaminobenzidine (DAB) staining. The darker the brown precipitate is, the higher the ROS level