Fig. 4. Upregulated levels of α-synuclein and phospho-tau^{Ser202/Thr205} were observed in SN of VPS35^D620N/+ knockin mice at age of 16 months.

A, B Protein expression of cytosolic α-synuclein (α-Syn) or phospho-tau^{Ser202/Thr205} (p-Tau) was upregulated in SN of 16-month-old heterozygous VPS35^D620N/+ mice. Upregulated expression of α-synuclein or phospho-tau^{Ser202/Thr205} was not found in SN of VPS35^D620N/+ mice at age of 12 months. A similar protein level of cytosolic phospho-α-synuclein^Ser129 (p-α-Syn) or tau was found in SN of 12- or 16-month-old WT and VPS35^D620N/+ mice. **P < 0.01 compared with WT mice. Each bar represents mean ± S.E. value of seven mice. C Confocal double immunofluorescence staining showed that cytosolic expression of α-synuclein was significantly upregulated in TH-positive SNpc DAergic neurons of VPS35^D620N/+ knockin mice at age of 16 months. *P < 0.05 compared with WT mice. Each bar represents mean ± S.E. value of 80 neurons from four mice. D Immunocytochemical staining using anti-phospho-tau^{Ser202/Thr205} antiserum showed that tau pathology-like protein aggregates of phospho-tau (arrow) were found in SN of VPS35^D620N/+ mice aged 16 months. Scale bar is 30 μm.