Table 2.
Primers used for relative real-time PCR.1
| Gene name | Primers sequence (5′-3′) | GenBank accession |
|---|---|---|
| β-actin | F: TGAGAGTAGCCCCTGAGGAGCAC | NM_001310421.1 |
| R: TAACACCATCACCAGACTCCATCAC | ||
| ZO-1 | F: CACAGCAGCCCAGCAACGG | XM_027465580.1 |
| R: TCGCCTGCCACCTCTTCCATAG | ||
| CLDN-1 | F: AGCCTTCCTCTGCTGCTCCTG | XM_013108556.3 |
| R: AGTGCTGGTGGCTCCTCATCC | ||
| CLDN-2 | F: TCGCCTACTCCAACGCCTACC | XM_021271062.2 |
| R: GGGTTTGTGTGTCGGGCTGAC |
Abbreviations: CLDN-1, claudin-1; CLDN-2, claudin-2; F, forward; R, reverse; ZO-1, zonula occludens protein-1.
1
Primers designed by primer express software (Applied Biosystems, Foster City, CA).