Figure 5.

Coimmunoprecipitation (co-IP) analysis of interaction between PPARγ isoforms and Tip60. Co-immunoprecipitation analysis of the PPARγ isoforms and Tip60. ICP2 cells were transfected with the indicated expression vectors (pCMV-HA-PPARγ1, pCMV-HA-PPARγ2, pCMV-Myc-Tip60, and pCMV-Myc-RXRα). Cells were harvested and lysed in western and IP buffer, and Tip60 and RXRα were immunoprecipitated (IP) using anti-HA. The precipitated proteins were subjected to Western blot analysis to detect Myc-RXRα and Myc-Tip60 using Anti-Myc. An aspecific band is indicated (∗). Abbreviations: ICP2, immortalized chicken preadipocytes; PPARγ, peroxisome proliferator-activated receptor γ; Tip60, Tat-interacting protein 60.