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. 2020 Oct 9;99(12):6619–6629. doi: 10.1016/j.psj.2020.09.088

Table 3.

Effect of a higher eggshell temperature (EST) of 38.9°C during week 2 of incubation (Higher) and/or a lower EST of 36.7°C during week 3 of incubation (Lower) compared with a control EST of 37.8°C (Control) and sex on Newcastle disease (NCD) titer of broilers at hatch, at day 22 (7 d after NCD vaccination), and slaughter age (day 41 or 42).

NCD titer
Treatment Hatch1 (log10titer) Day 222 (log10titer) Slaughter2 (log10titer)
EST week 2
 Control 3.2 1.5 2.6b
 Higher 3.1 1.7 2.8a
 SEM 0.1 0.1 0.1
EST week 3
 Lower 3.0 1.5 2.8
 Control 3.2 1.7 2.7
 SEM 0.1 0.1 0.1
EST week 2 × week 3
 Control × Lower 3.0 1.4 2.7
 Control × Control 3.3 1.6 2.6
 Higher × Lower 3.0 1.6 2.8
 Higher × Control 3.1 1.7 2.8
 SEM 0.1 0.2 0.1
P-value
 Week 2 0.38 0.41 0.02
 Week 3 0.15 0.50 0.08
 Week 2 × week 3 0.58 0.79 0.82
 Sex 0.28 0.60 0.02
1

n = 13, 12, 12, 12 for treatments Control x Lower, Control× Control, Higher × Lower, Higher × Control, respectively.

2

n = 16/treatment.