. 2020 Oct 2;99(12):6569–6575. doi: 10.1016/j.psj.2020.09.054

Table 4.

List of primers used in qRT-PCR for target gene mRNA detection in chickens.

Primer	Sequence 5′–3′	Annealing temperature/time	References
IgA Fw	GTCACCGTCACCTGGACTACA	59°C/30 s	Lammers et al., 2010
IgA Rev	ACCGATGGTCTCCTTCACATC	59°C/30 s	Lammers et al., 2010
MUC-2 Fw	GCTGATTGTCACTCACGCCTT	54°C/1 min	Smirnov et al., 2006
MUC-2 Rev	ATCTGCCTGAATCACAGGTGC	54°C/1 min	Smirnov et al., 2006
IGF-2 Fw	CTCTGCTGGAAACCTACTGT	55°C/30 s	Mudroňová et al., 2018
IGF-2 Rev	GAGTACTTGGCATGAGATGG	55°C/30 s	Mudroňová et al., 2018
GAPDH Fw	CCTGCATCTGCCCATTT	59°C/30 s	De Boever et al., 2008
GAPDH Rev	GGCACGCCATCACTATC	59°C/30 s	De Boever et al., 2008

Abbreviation: qRT-PCR, quantitative real-time PCR.